Author Topic: Morchella spp.  (Read 10895 times)

Offline NSF

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Re: Morchella spp.
« Reply #30 on: January 22, 2012, 07:26:27 PM »
I can't wait to see what trials you come up with and i'm very keen to see you succeed!  Best of luck, may the sclerotia be with you!

Offline BigLaughingJim

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Re: Morchella spp.
« Reply #31 on: January 22, 2012, 09:21:00 PM »
 Designing the trials tonight. Now, should I keep you in suspense and hide all the details in case I decipher the morchella code?
I'm going off my own path on this one. I don't trust the patent or the believe that the concepts presented in it are sound. Maybe I'm digging a hole that will one day be my grave, or maybe I'm tunneling to china....you'll b notified either way.
Again, wish me luck
jim@aufungi.com
go within, or you go without

Offline BigLaughingJim

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Re: Morchella spp.
« Reply #32 on: January 23, 2012, 03:50:25 PM »
Trial is officially on. I'm considering starting up a Morchella trial thread and keeping everyone updated on my every move, but I dont want to dissapoint so I may just stick up some progress posts here...
Just a little insight into the design
8 blocks, 3 replicates per block, 500g sample unit per replicate
constants will be the growth substrate the culture and the base casing mixture
variables will be 3 monoculture microflora additions to the casing layer and 4 combinations of the monocultures, control being no microflora
All 500g replicate units have been prepared and inoculated as of today, with an additional 4 units incase some contams appear.
all for now.
jim@aufungi.com
go within, or you go without

Offline worowa

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Re: Morchella spp.
« Reply #33 on: January 23, 2012, 04:54:27 PM »
If you've got a spare petri culture, I could knock up 50 or so plates and spread them around for a group experiment?

Offline BigLaughingJim

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Re: Morchella spp.
« Reply #34 on: January 23, 2012, 05:11:44 PM »
Hands up, Who would participate in a group experiment?
I will gladly donate the culture, though I am not sure what species it is. I believe the parent fruiting body was a yellow morel but the drying process and other factors could have made a grey morel look yellow. Regardless, its a new strain captured from the wild in South Africa. VERY vigorous, as you wuold expect a morel to be.

If you want to participate, I want to see your ideas for experiments. What factors will you hold as constants and what variables? What will be your measured approach and how will you make your experiment uniform, documented and repeatable? Furthermore, anyone who strikes it rich using this strain will pay me royalties..or will at least fund my yearly trips to ISMS conferences!
jim@aufungi.com
go within, or you go without

Offline absinthe_cat

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Re: Morchella spp.
« Reply #35 on: February 10, 2012, 08:37:41 PM »
I got six plates all with some very purple mycelium and odor is very sanitizer_like smelling of a hospital. The purple is only evident after a few days as the mycelium is actually very white. The purple is not sporultaion tho...so I'm stumped as to what I actually cultered there.

this is very curious.  im keen to know more.

im assuming you didsome  microscopy in order to dtermine what was going in.

is the mycelium purple, or has it transitioned from white to purple?

Cheers, Absinthe_cat.
« Last Edit: May 12, 2012, 05:08:24 PM by absinthe_cat »

Offline BigLaughingJim

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Re: Morchella spp.
« Reply #36 on: February 11, 2012, 09:37:10 AM »
Obtuse,
White, brilliant mycelium. Fully colonizes a substrate and them some lavendar metabolites show up. On the plates the myc colonizes normally white then all shades purple. Its not sporulation. I haven't put it under the scope, because I haven't been compelled to do so. I am certain whatever it is is interesting, and could be a resonable addition to a fruiting media for morchella because of the position I discovered it in was on the internal walls of the fruiting bodies( no chance of ever being exposed to air outside). Maybe its an obligate parasite, which influences the sporocarp formation in order to feeed on some portion of it.
Yes I still have cultures and spawn, was too interesting to purge.
jim@aufungi.com
go within, or you go without

Offline absinthe_cat

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Re: Morchella spp.
« Reply #37 on: February 29, 2012, 09:25:49 AM »
Any ideas on what the purple metabolite could be.

very curious  ???

Cheers, Absinthe_cat.
« Last Edit: May 12, 2012, 05:07:10 PM by absinthe_cat »

Offline absinthe_cat

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Re: Morchella spp.
« Reply #38 on: April 15, 2012, 11:57:29 AM »
Hi all,

any idea what this is: (see attached image)

There is a whole heap of them on my old morel plate.  the rest of the plate looks dead and done.

I transferred a few to agar, and they had the most insanely fluffy aerial hyphae grow from them.  the hyphae grow for a couple of cm and then become aerial.

no new sclerotia forming yet.

all very wierd and im waiting to see if it is a contaminant.

Cheers, Absinthe_cat.
« Last Edit: May 12, 2012, 05:06:19 PM by absinthe_cat »

Offline NSF

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Re: Morchella spp.
« Reply #39 on: April 15, 2012, 09:25:59 PM »
No idea but i hope they're conidia!

Offline yanmaneee

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